ADRA2A Antikörper (Extracellular)

Produktnummer ABIN7042899

Produktdetails anti-ADRA2A Antikörper

Target: ADRA2A (Alpha-2A Adrenergic Receptor (ADRA2A))

Bindungsspezifität: AA 7-20, Extracellular

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ADRA2A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunofluorescence (Cultured Cells) (IF (cc))

Verwendungszweck: A Rabbit Polyclonal Antibody to α2A-Adrenergic Receptor

Spezifität: Extracellular, N-terminus

Kreuzreaktivität: Maus, Ratte

Homologie: Mouse - identical, human - 13,14 amino acid residues identical

Produktmerkmale: Anti-α2A-Adrenergic Receptor (extracellular) Antibody (ABIN7042899, ABIN7043918 and ABIN7043919)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize α2A-adrenoceptor from mouse, rat, and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DAGNSSWNGTEAPG, corresponding to amino acid residues 7-20 of rat alpha2A-adrenoceptor

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:250

Application Dilutions Western blot wb: 1:200

Kommentare:

Cited Application: IHC

Negative Control: BLP-AR020

Blocking Peptide: BLP-AR020

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu ADRA2A

Target: ADRA2A (Alpha-2A Adrenergic Receptor (ADRA2A))

Andere Bezeichnung: ADRA2A (ADRA2A Produkte)

Hintergrund: Alpha-2A adrenoceptor, Alpha-2D adrenergic Receptor, α-2AAR, ADA2A, ADRA2A,Adrenergic receptors (also called adrenoceptors) are the receptors for the catecholamines adrenaline and noradrenaline (called epinephrine and norepinephrine in the United States). Adrenaline and noradrenaline play important roles in the control of blood pressure, myocardial contractile rate and force, airway reactivity, and a variety of metabolic and central nervous system functions. Adrenergic receptors are members of the G-protein coupled receptor (GPCR) superfamily of membrane proteins. They share a common structure of seven putative transmembrane domains, an extracellular N-terminus, and a cytoplasmic C- terminus.Adrenoceptors are divided into three types: α1, α2 and β-adrenoceptors. Each type is further divided into at least three subtypes: α1A, α1B, α1D, α2A, α2B, α2C, β1, β2, β31,2. Adrenoceptors are expressed in nearly all peripheral tissues and in the central nervous system1,2. In general, the pharmacological properties of each subtype are quite homogenous across different species. However, this is not the case for the α2A subtype which was first isolated from human platelets3. This subtype shows different pharmacological properties from that of mouse and rat. For this reason, until molecular techniques significantly advanced, it was believed that human α2A and that of mouse and rat (termed α2D) were two different subtypes. Today, it is accepted that these two subtypes are in fact one gene product and is generally termed α2A. One of the main differences between this subtype from the different organisms is its affinity for yohimbine and rauwolscine, rat and mouse α2A displays less affinity compared to human3,4.

Alternative names: alpha2A-Adrenergic Receptor, Alpha-2A adrenoceptor, Alpha-2D adrenergic Receptor, alpha-2AAR, ADA2A, ADRA2A

Gen-ID: 25083

NCBI Accession: NM_000681

UniProt: P22909

Pathways: EGFR Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Negative Regulation of Transporter Activity