ADRA1B Antikörper (Extracellular)

Produktnummer ABIN7042897

Produktdetails anti-ADRA1B Antikörper

Target: ADRA1B (Adrenergic, alpha-1B-, Receptor (ADRA1B))

Bindungsspezifität: AA 21-35, Extracellular

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ADRA1B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (IF), Live Cell Imaging (LCI), Immunochromatography (IC)

Verwendungszweck: A Rabbit Polyclonal Antibody to α1B-Adrenergic Receptor

Spezifität: Extracellular, N-terminus

Kreuzreaktivität: Human, Maus, Ratte

Homologie: 15 amino acid residues identical, rat - 13,Mouse - 14

Produktmerkmale: Anti-α1B-Adrenergic Receptor (extracellular) Antibody (ABIN7042897, ABIN7043914 and ABIN7043915)) is a highly specific antibody directed against an extracellular epitope of the human α1B-adrenoceptor. The antibody can be used in western blot, immunohistochemistry, live cell imaging and indirect flow cytometry applications. It has been designed to recognize α1B-adrenoceptor from human, rat and mouse samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KNANFTGPNQTSSNS, corresponding to amino acid residues 21-35 of human alpha1B-adrenoceptor

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Kommentare:

Cited Application: IHC

Negative Control: BLP-AR018

Blocking Peptide: BLP-AR018

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu ADRA1B

Target: ADRA1B (Adrenergic, alpha-1B-, Receptor (ADRA1B))

Andere Bezeichnung: ADRA1B (ADRA1B Produkte)

Hintergrund: Alpha-1B adrenoceptor, ADA1B, ADRA1B,Adrenergic receptors (also called adrenoceptors) are the receptors for the catecholamines adrenaline and noradrenaline (called epinephrine and norepinephrine in the United States). Adrenaline and noradrenaline play important roles in the control of blood pressure, myocardial contractile rate and force, airway reactivity, and a variety of metabolic and central nervous system functions. Adrenergic receptors are members of the G-protein coupled receptor (GPCR) superfamily of membrane proteins. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.Adrenoceptors are divided into three types: α1, α2 and β adrenoceptors. Each type is further divided into at least three subtypes: α1A, α1B, α1D, α2A, α2B, α2C, β1, β2, β3.1,2 They are expressed in nearly all peripheral tissues and in the central nervous system.1,2 All α1-adrenoceptors (α1-ARs) activate phospholipases C and A2.3 In addition to mobilizing intracellular calcium, the α1-ARs have also been shown to activate calcium influx via voltage-dependent and -independent calcium channels.4 α1B-Adrenergic receptor populations are greatest in the spleen, kidney, cerebellum, and fetal brain.5α1B-Adrenoceptor causes contraction of smooth muscle cells and thereby controls vascular tone, blood pressure, and accelerates the development of atherosclerosis.5

Alternative names: alpha1B-Adrenergic Receptor, Alpha-1B adrenoceptor, ADA1B, ADRA1B

Gen-ID: 147

NCBI Accession: NM_000679

UniProt: P35368

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process