ADRA1D Antikörper (C240S, Extracellular)

Produktnummer ABIN7042896

Produktdetails anti-ADRA1D Antikörper

Target: ADRA1D (Adrenergic Receptor, alpha 1d (ADRA1D))

Bindungsspezifität: AA 231-245, C240S, Extracellular

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ADRA1D Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunochromatography (IC), Live Cell Imaging (LCI)

Verwendungszweck: A Rabbit Polyclonal Antibody to α1D-Adrenergic Receptor

Spezifität: 3rd extracellular loop

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Rat,human,mouse - 14,15 amino acid residues identical

Produktmerkmale: Anti-alpha1D-Adrenergic Receptor (extracellular) Antibody is directed against an extracellular epitope of the rat α1D-adrenergic receptor. Anti-α1D-Adrenergic Receptor (extracellular) Antibody (ABIN7042896, ABIN7043916 and ABIN7043917) can be used in western blot, indirect flow cytometry and immunohistochemical applications. It has been designed to recognize α1D-adrenoceptor from mouse, rat and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EPVPPDERF*SGITEE, corresponding to amino acid residues 231-245 of rat alpha1D-adrenoceptor with replacement of cysteine 240 (C240) with serine (*S)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Kommentare:

Negative Control: (ABIN7234657)

Blocking Peptide: (ABIN7234657)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu ADRA1D

Target: ADRA1D (Adrenergic Receptor, alpha 1d (ADRA1D))

Andere Bezeichnung: ADRA1D (ADRA1D Produkte)

Hintergrund: Alpha-1D adrenoceptor, Alpha-1D adrenoreceptor, ADRA1D,Adrenergic receptors (also called adrenoceptors) are the receptors for the catecholamines adrenaline and noradrenaline (called epinephrine and norepinephrine in the United States). Adrenaline and noradrenaline play important roles in the control of blood pressure, myocardial contractile rate and force, airway reactivity, and a variety of metabolic and central nervous system functions. Adrenergic receptors are members of the G-protein coupled receptor (GPCR) superfamily of membrane proteins. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.Adrenoceptors are divided into three types: α1, α2 and β-adrenoceptors. Each type is further divided into at least three subtypes: α1A, α1B, α1D, α2A, α2B, α2C, β1, β2, β31,2. The adrenoceptors are expressed in nearly all peripheral tissues and in the central nervous system1,2. α1-Adrenergic receptors play an important role in the physiological response to epinephrine and norepinephrine, particularly in the cardiovascular system. All three cloned α1 receptors (α1A, α1B, and α1D) couple to Gq/11. While their cellular distribution is clear, their functional role is less so due to a lack of specific agonists/antagonists3. As these receptors lack specific agonists/antagonists, knock-out (KO) studies in mice have shed significant light on their cellular role. Individual α1-adrenoceptor KO mice do not display physical abnormalities in general. Similar KO studies indicate that all α1 subtypes seem to be involved in the regulation of blood pressure3. Also, studies indicate that α1D-adrenoceptor seems to play an important role in aortic contraction4, renal function, nociception, basal locomotor activity3 as well as bladder contraction5.

Alternative names: alpha1D-Adrenergic Receptor, Alpha-1D adrenoceptor, Alpha-1D adrenoreceptor, ADRA1D

Gen-ID: 29413

NCBI Accession: NM_000678

UniProt: P23944