Histone 3 Antikörper (H3K36me2)

Produktnummer ABIN703841

Der Kaninchen Polyklonal Anti-Histone 3-Antikörper wurde für ELISA, ICC, IHC (p), FACS, IF (cc), IF (p) und IHC (fro) validiert. Er ist geeignet, Histone 3 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für Histone 3 Antikörper (H3K36me2) (ABIN703841)

Target: Histone 3 (H3) (Histone H3 (H3))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Histone 3 Antikörper ist unkonjugiert

Applikation: ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-Histone 3 Antikörper

Bindungsspezifität: H3K36me2

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Cow,Pig,Rabbit,Drosophila

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic metylpeptide derived from human Histone H3 around metylation site of Di Methyl Lys36

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Andere Bezeichnung: Histone H3

Hintergrund:

Synonyms: Histone H3Di Methyl Lys36, Di methyl Histone H3Lys36, Di methyl Histone H3K36, H3 histone family member E pseudogene, H3F3, HIST3H3, Histone H3 3 pseudogene, H31_TETTH, Histone H3, H3S, Histone H3-I/H3-II, Major histone H3, H3F, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l.

Background: Modulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranslational modifications including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression. In most species, the histone H2B is primarily acetylated at lysines 5, 12, 15 and 20. Histone H3 is primarily acetylated at lysines 9, 14, 18 and 23. Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis.

Gen-ID: 3020