Tumor Protein p73 Antikörper (pTyr99) (HRP)

Produktnummer ABIN703114

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Tumor Protein p73 in WB, ELISA, IHC (p) und IHC (fro). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Tumor Protein p73 Antikörper (pTyr99) (HRP) (ABIN703114)

Target: Tumor Protein p73 (TP73) (Tumor Protein P73 (TP73))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Tumor Protein p73 Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-Tumor Protein p73 Antikörper (HRP)

Bindungsspezifität: pTyr99

Kreuzreaktivität: Human

Homologie: Mouse,Rat

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human p73 around the phosphorylation site of Tyr99 [SP(p-Y)AQ]

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handhabung: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu Tumor Protein p73

Target: Tumor Protein p73 (TP73) (Tumor Protein P73 (TP73))

Andere Bezeichnung: p73

Hintergrund:

Synonyms: p73Tyr99, TP73phospho Y99, p53 like transcription factor, p53 Related Protein, p73, TP 73, TP73, Tumor Protein p73, P73_HUMAN.

Background: P73 protein is a structural and functional homologue of p53, a tumor suppressor gene. In this study, The p73 protein, p19ras, by the yeast two-hybrid screening method. Alternative splicing of the proto-oncogene H-ras pre-mRNA has led to two distinct transcripts, Ras proteins are known to be small membrane-localized guanine nucleotide-binding proteins. However, unlike other Ras proteins, p19ras is localized in the nucleus and the cytosol and its interaction with P73 protein occurred exclusively in the nucleus. Oncogenic MDM2 (mouse double minutes 2) is a known repressor of p73 transcriptional activity. In this study, when p19ras was bound to MDM2, it further inhibited the association of MDM2 to the p73 protein. Therefore, this study presents a novel pathway of Ras signaling that occurs in the nucleus, involving p19ras and p73.

Gen-ID: 7161

Pathways: Regulation of Cell Size, Positive Regulation of Response to DNA Damage Stimulus