KEAP1 Antikörper (AA 65-160)

Produktnummer ABIN702085

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch KEAP1 in WB, ELISA, IHC (p), IF (cc), IF (p) und IHC (fro). Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Schwein und Kaninchen und wurde in 5+ Publikationen erwähnt.

Kurzübersicht für KEAP1 Antikörper (AA 65-160) (ABIN702085)

Target: KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))

Reaktivität: Human, Maus, Ratte, Schwein, Kaninchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser KEAP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-KEAP1 Antikörper

Bindungsspezifität: AA 65-160

Kreuzreaktivität: Human, Maus, Schwein, Kaninchen, Ratte

Homologie: Dog,Cow

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human KEAP1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-KEAP1 Antikörper (ABIN702085)

Su, Liu, Wang, Wang, Song, Song: "The electrophilic character of quinones is essential for the suppression of Bach1." in: Toxicology, Vol. 387, pp. 17-26, (2017) (PubMed).

Kouam, Yuan, Njayou, He, Tsayem, Oladejo, Song, Moundipa, Gao: "Induction of Mkp-1 and Nuclear Translocation of Nrf2 by Limonoids from Khaya grandifoliola C.DC Protect L-02 Hepatocytes against Acetaminophen-Induced Hepatotoxicity." in: Frontiers in pharmacology, Vol. 8, pp. 653, (2017) (PubMed).

Chen, Lin, Liu, Hsu, Lin, Su: "Caveolin-1 Expression Ameliorates Nephrotic Damage in a Rabbit Model of Cholesterol-Induced Hypercholesterolemia." in: PLoS ONE, Vol. 11, Issue 4, pp. e0154210, (2016) (PubMed).

Su, Zhang, Song, Shi, Fu, Xia, Bai, Hu, Xu, Song, Song: "Tetrachlorobenzoquinone activates nrf2 signaling by keap1 cross-linking and ubiquitin translocation but not keap1-cullin3 complex dissociation." in: Chemical research in toxicology, Vol. 28, Issue 4, pp. 765-74, (2015) (PubMed).

Su, Xia, Shi, Song, Fu, Xiao, Chen, Lu, Sun, Wu, Yang, Li, Ye, Song, Song: "Neohesperidin Dihydrochalcone versus CCl?-Induced Hepatic Injury through Different Mechanisms: The Implication of Free Radical Scavenging and Nrf2 Activation." in: Journal of agricultural and food chemistry, Vol. 63, Issue 22, pp. 5468-75, (2015) (PubMed).

Details zu KEAP1

Target: KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))

Andere Bezeichnung: KEAP1

Hintergrund:

Synonyms: INrf2, KLHL19, Kelch-like ECH-associated protein 1, Cytosolic inhibitor of Nrf2, Kelch-like protein 19, KEAP1, KIAA0132

Background: Acts as a substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1 and targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. Retains NFE2L2/NRF2 and may also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome.

Gen-ID: 9817

UniProt: Q14145

Pathways: Maintenance of Protein Location