GATA1 Antikörper (C-Term)

Produktnummer ABIN7013869

Der Ziege Polyklonal Anti-GATA1 Antikörper wurde für WB, ELISA, IF und FACS validiert. Er ist geeignet, GATA1 in Proben von Human zu detektieren.

Kurzübersicht für GATA1 Antikörper (C-Term) (ABIN7013869)

Target: GATA1 (GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser GATA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-GATA1 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: GATA1

Sequenz: PTTSTTVVAP LSS

Kreuzreaktivität: Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 50 kDa band observed in nuclear lysates of cell line K562 (calculated MW of 42.8 kDa according to NP_002040.1). The observed molecular weight corresponds to different antibodies from other sources and was successfully blocked by incuba

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu GATA1

Target: GATA1 (GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1))

Andere Bezeichnung: GATA1

Hintergrund: GATA1, GATA binding protein 1 (globin transcription factor 1), GF1, NFE1, ERYF1

Gen-ID: 2623

Pathways: Cellular Response to Molecule of Bacterial Origin