EDF1 Antikörper (N-Term)

Produktnummer ABIN7013866

Der Ziege Polyklonal Anti-EDF1-Antikörper wurde für WB, IHC, ELISA, IF und FACS validiert. Er ist geeignet, EDF1 in Proben von Human und Maus zu detektieren.

Kurzübersicht für EDF1 Antikörper (N-Term) (ABIN7013866)

Target: EDF1 (Endothelial Differentiation Related Factor 1 (EDF1))

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser EDF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-EDF1 Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: EDF1 / MBF1

Sequenz: AESDWDTVTV LRK

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Pancreas and Brain (Cortex). Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 19 kDa band observed in nuckear lysates of cell line HEK293, approx. 18 kDa in nuckear lysates of cell line HeLa, and approx 17 kDa in nuckear lysates of cell line NIH3T3 (calculated MW of 16.4 kDa according to Human NP_003783.1 and Mous

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the nuclei of A431 and HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10u

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu EDF1

Target: EDF1 (Endothelial Differentiation Related Factor 1 (EDF1))

Andere Bezeichnung: EDF1

Hintergrund: EDF1, MBF1, EDF-1, MGC9058, endothelial differentiation-related factor 1, multiprotein bridging factor 1

Gen-ID: 8721, 59022