SCRIB Antikörper (Internal Region)

Produktnummer ABIN7013862

Dieser Ziege Polyklonal Antikörper erkennt spezifisch SCRIB in WB, ELISA und IF. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für SCRIB Antikörper (Internal Region) (ABIN7013862)

Target: SCRIB (Scribbled Homolog (SCRIB))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser SCRIB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-SCRIB Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: SCRIB / Scribble

Sequenz: PEGPGKEKEL PGQ

Spezifität: This antibody is expected to recognize both reported isoforms (NP_874365.3, NP_056171.3).

Kreuzreaktivität: Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 200-250 kDa band observed in lysates of cell lines HeLa, HepG2 and U2OS (calculated MW of 178 kDa according to NP_874365.3). This molecular weight is routinely observed by other sources. Recommended concentration: 1-3 μg/mL. Primary inc

Peptide ELISA: antibody detection limit dilution 1:1000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cell junctions of MCF7 cells. Recommended concentration: 10μg/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu SCRIB

Target: SCRIB (Scribbled Homolog (SCRIB))

Andere Bezeichnung: SCRIB

Hintergrund: SCRIB, scribbled homolog (Drosophila), CRIB1, SCRB1, SCRIB1, Vartul, scribble

Gen-ID: 23513

Pathways: Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Tube Formation, Synaptic Vesicle Exocytosis, Asymmetric Protein Localization