DLAT Antikörper

Produktnummer ABIN7257932

Der Kaninchen Polyklonal Anti-DLAT-Antikörper wurde für WB, IHC und IF validiert. Er ist geeignet, DLAT in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für DLAT Antikörper (ABIN7257932)

Target: DLAT (Dihydrolipoyl Transacetylase (DLAT))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DLAT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-DLAT Antikörper

Produktmerkmale: Polyclonal Antibody

Aufreinigung: Affinity purification

Immunogen: Recombinant fusion protein of human DLAT (NP_001922.2).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000 IHC 1:50-1:100 IF 1:50-1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze / thaw cycles.

Details zu DLAT

Target: DLAT (Dihydrolipoyl Transacetylase (DLAT))

Andere Bezeichnung: DLAT

Hintergrund: This gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95 % of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood.

Molekulargewicht:

Observed_MW: 69 kDa

Calculated_MW: 68 kDa

Gen-ID: 1737

UniProt: P10515