Histone H4 Antikörper (AA 2-103)

Produktnummer ABIN7440314

Dieser unabhängig validierte Kaninchen Polyklonal Antikörper erkennt spezifisch Histone H4 in WB, IHC, ICC und IP. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Schwein, Rind (Kuh), Huhn, Cat, Hund, Meerschweinchen, Pferd und Rhesusaffen.

Kurzübersicht für Histone H4 Antikörper (AA 2-103) (ABIN7440314)

Target: Histone H4

Reaktivität: Human, Maus, Ratte, Schwein, Rind (Kuh), Huhn, Cat, Hund, Meerschweinchen, Pferd, Rhesusaffen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Histone H4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Produktdetails anti-Histone H4 Antikörper

Bindungsspezifität: AA 2-103

Verwendungszweck: Polyclonal Antibody to Histone H4 (H4)

Spezifität: The antibody is a rabbit polyclonal antibody raised against H4. It has been selected for its ability to recognize H4 in immunohistochemical staining and western blotting.

Kreuzreaktivität: Cat, Huhn, Rind (Kuh), Hund, Meerschweinchen, Pferd, Human, Maus, Schwein, Kaninchen, Ratte, Rhesusaffen

Aufreinigung: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Histone H4 (H4) corresdonding to Ser2~Gly103 with N-terminal His and GST Tag

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Kommentare:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Independent Validation (IHC)

Validierung #104631 (Immunohistochemistry)

by: Prof. Merighi, Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin

No.: #104631

Datum: 04.08.2025

Antigen: Histone H4

Chargennummer: A20250716116

Validierte Anwendung: Immunohistochemistry

Positivkontrolle:

Negativkontrolle:

Control slices were processed for each experimental procedure, omitting the primary antibody. In this case, overnight incubation was only performed using a diluent solution.

Validierungsbilder

Histone H4 immunoreactive cells in the cerebral cortex and hippocampus of an adult CD1 mouse. Antibody dilution: 1:25. Magnification: 63x.

Protokoll

Primärantikörper: ABIN7440314

Sekundärantikörper: anti-rabbit Alexa Fluor 488 (Invitrogen A11008, lot # 3112790)

Full Protocol:

• Sample analyzed: adult (9 months old) CD1 mouse brain.
• Paraffin-embedded slices (7µm thick) were deparaffinized and rehydrated through a graded series of alcohols and distilled water (dH2O).
• Sections were blocked in ovalbumin 1% 1 hour at room temperature.
• Antigen retrieval was performed in all the sections by microwave treatment (1 minute at 750W + 1 minute at 250W in Citrate buffer pH 6).
• 3x5 minutes washing in 0.01M PBS.
• Sections were incubated with the primary antibody at the following dilutions of 1:25/1:50/1:100/1:200, overnight at room temperature (primary antibody was diluted in PLL/BSA/PBS diluent).
• 3x5 minutes washing in 0.01M PBS.
• Incubation with the anti-rabbit secondary antibody 1:400 in PLL/BSA/PBS diluent, 1 hour at room temperature.
• 3x5 minutes washing in 0.01M PBS.
• Nuclear counterstaining was performed with 4′,6-Diamidine-2′-phenylindole dihydrochloride (DAPI) 1µg/mL, 2 minutes.
• 2x5 minutes washing in 0.01M PBS.
• Specimens were then mounted in Fluoroshield mounting medium (Sigma).
• Images were acquired with a Leica DM 6000B fluorescence microscope with a digital camera at 63x magnification.

Anmerkungen:

Passed. The antibody works in IHC-P at concentrations from 1:25-1:50.

Handhabung

Format: Liquid

Konzentration: 0.33 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 24 months

Details zu Histone H4

Target: Histone H4