Nestin Antikörper (AA 178-399)

Produktnummer ABIN7426685

Der Maus Monoklonal Anti-Nestin-Antikörper wurde für WB, IHC, ICC und IP validiert. Er ist geeignet, Nestin in Proben von Human zu detektieren.

Kurzübersicht für Nestin Antikörper (AA 178-399) (ABIN7426685)

Target: Nestin (NES)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Nestin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Klon: D10

Produktdetails anti-Nestin Antikörper

Bindungsspezifität: AA 178-399

Verwendungszweck: Monoclonal Antibody to Nestin (NES)

Spezifität: The antibody is a mouse monoclonal antibody raised against NES. It has been selected for its ability to recognize NES in immunohistochemical staining and western blotting.

Kreuzreaktivität: Ratte

Aufreinigung: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Nestin (NES) corresdonding to Leu178~Pro399 with N-terminal His Tag

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Flow cytometry: 10 μg/mL,Optimal working dilutions must be determined by end user.

Kommentare:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 24 months

Details zu Nestin

Target: Nestin (NES)

Andere Bezeichnung: Nestin

UniProt: P48681