DIRAS3 Antikörper (C-Term)

Produktnummer ABIN6992120

Der Kaninchen Polyklonal anti-DIRAS3 Antikörper wird verwendet zum Nachweis von DIRAS3 in Proben von Human. Er wurde validiert für WB und ELISA.

Kurzübersicht für DIRAS3 Antikörper (C-Term) (ABIN6992120)

Target: DIRAS3 (DIRAS Family, GTP-Binding RAS-Like 3 (DIRAS3))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DIRAS3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-DIRAS3 Antikörper

Bindungsspezifität: C-Term

Spezifität: DIRAS3 antibody is human specific.

Aufreinigung: DIRAS3 antibody is affinity chromatography purified via peptide column.

Immunogen: DIRAS3 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human DIRAS3. The immunogen is located within the last 50 amino acids of DIRAS3.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: DIRAS3 antibody can be used for detection of DIRAS3 by Western blot at 1 - 2 μ,g/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: DIRAS3 antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: DIRAS3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Details zu DIRAS3

Target: DIRAS3 (DIRAS Family, GTP-Binding RAS-Like 3 (DIRAS3))

Andere Bezeichnung: DIRAS3

Hintergrund: DIRAS3 is a member of the ras superfamily, and is expressed in normal ovarian and breast epithelial cells, but not in ovarian and breast cancers. It is an imprinted gene, with mono-allelic expression of the paternal allele, which is associated with growth suppression and down-regulation of cyclin D1 promoter activity and induction of p21 (WAF/CIP1). Thus, this gene appears to be a putative tumor suppressor gene whose function is abrogated in ovarian and breast cancers (1). DIRAS3 has been shown to induce autophagy in human ovarian cancer cells by blocking PI3K signaling, inhibiting the mammalian target of rapamycin (TOR), upregulating ATG4, and colocalizing with LC3 in autophagosomes (2). DIRAS also interacts with C-RAF and downregulates mitogen-activated protein kinase kinases (MEK) to restrict cell migration (3).

Molekulargewicht: Predicted: 25 kDa

Observed: 24 kDa

Gen-ID: 9077

NCBI Accession: NP_004666

UniProt: O95661