DTX4 antibody is human and mouse reactive. At least two isoforms of DTX4 are known to exist, this antibody will detect both isoforms. DTX4 antibody is predicted to not cross-react with other DTX protein family members.
Aufreinigung
DTX4 antibody is affinity chromatography purified via peptide column.
Immunogen
DTX4 antibody was raised against a 19 amino acid peptide near the center of human DTX4. The immunogen is located within amino acids 230 - 280 of DTX4.
DTX4
Reaktivität: Human
ELISA, WB
Wirt: Kaninchen
Polyclonal
FITC
Applikationshinweise
DTX4 antibody can be used for detection of DTX4 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1 mg/mL
Buffer
DTX4 antibody is supplied in PBS containing 0.02 % sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C,4 °C
Informationen zur Lagerung
DTX4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
The Deltex 4 E3 ubiquitin ligase (DTX4) is a member of a protein family that is involved in Notch signaling and neurogenesis (1). Recent studies have shown that DTX4 is also associated with the innate immune response (2). Following the activation of type I interferon signaling, the Nod-like receptor protein 4 (NLRP4) recruits DTX4 for Lys48-linked polyubiquitination and degradation of the kinase TBK1 (NAK), thereby maintaining immune homeostasis during antiviral innate immunity (2).