MIB1 Antikörper (C-Term)

Produktnummer ABIN6992000

Der Kaninchen Polyklonal Anti-MIB1-Antikörper wurde für ELISA, WB, ICC und IF validiert. Er ist geeignet, MIB1 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für MIB1 Antikörper (C-Term) (ABIN6992000)

Target: MIB1 (Mindbomb E3 Ubiquitin Protein Ligase 1 (MIB1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MIB1 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Produktdetails anti-MIB1 Antikörper

Bindungsspezifität: AA 920-970, C-Term

Spezifität: MIB1 antibody is human, mouse and rat reactive. At least three isoforms of MIB1 are known to exist.

Aufreinigung: MIB1 antibody is affinity chromatography purified via peptide column.

Immunogen: MIB1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human MIB1. The immunogen is located within amino acids 920 - 970 of MIB1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: MIB1 antibody can be used for detection of MIB1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunocytochemistry at 5 μ,g/mL. For Immunoflorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: MIB1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: MIB1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Details zu MIB1

Target: MIB1 (Mindbomb E3 Ubiquitin Protein Ligase 1 (MIB1))

Andere Bezeichnung: MIB1

Hintergrund: The E3 ubiquitin-protein ligase MIB1, also known as Mindbomb homolog 1, contains multiple ankyrin repeats and RING finger domains that functions as an E3 ubiquitin ligase (1). MIB1 interacts with and regulates the cellular expression of the death-associated protein kinase 1 (DAPK1) protein by promoting its ubiquitination and degradation (1). It also positively regulates Notch signaling by ubiquitinating the Notch receptors, thereby facilitating their endocytosis (2).

Molekulargewicht: Predicted: 89, 102, 111 kDa

Observed: 103 kDa

Gen-ID: 57534

NCBI Accession: NP_065825

UniProt: Q86YT6

Pathways: SARS-CoV-2 Protein Interaktom, Phosphorylierungen bei SARS-CoV-2 Infektion