USP25 Antikörper (AA 470-520)

Produktnummer ABIN6991995

Der Kaninchen Polyklonal anti-USP25 Antikörper (ABIN6991995) detektiert spezifisch USP25 in WB, ELISA, IHC (p) und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für USP25 Antikörper (AA 470-520) (ABIN6991995)

Target: USP25 (Ubiquitin Specific Peptidase 25 (USP25))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser USP25 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Produktdetails anti-USP25 Antikörper

Bindungsspezifität: AA 470-520

Spezifität: USP25 antibody is human and mouse reactive. Multiple isoforms of USP25 are known to exist.

Aufreinigung: USP25 antibody is affinity chromatography purified via peptide column.

Immunogen: USP25 antibody was raised against a 17 amino acid peptide near the center of human USP25. The immunogen is located within amino acids 470 - 520 of USP25.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: USP25 antibody can be used for detection of USP25 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry at 5 μ,g/mL. For Immunoflorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: USP25 Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: USP25 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Details zu USP25

Target: USP25 (Ubiquitin Specific Peptidase 25 (USP25))

Andere Bezeichnung: USP25

Hintergrund: USP25 (ubiquitin specific peptidase 25), also known as USP21, belongs to the peptidase C19 family and is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response (1). It contains one UBA-like domain and two UIM (ubiquitin-interacting motif) repeats. Due to alternative splicing events, USP25 is expressed as two short, ubiquitously expressed isoforms and one long, muscle-specific isoform (2). The long isoform of USP25 (USP25m) is upregulated in myogenesis and is implicated in regulation of muscular differentiation and function. USP25 is a deubiquitinating enzyme (DUB) that negatively regulates IL-17-triggered signaling (3,4).

Molekulargewicht: Predicted: 116 kDa

Observed: 105 kDa

Gen-ID: 29761

NCBI Accession: NP_037528

UniProt: Q9UHP3