ME1 Antikörper (N-Term)

Produktnummer ABIN6991979

Der Kaninchen Polyklonal Anti-ME1-Antikörper wurde für WB, ELISA und IHC (p) validiert. Er ist geeignet, ME1 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für ME1 Antikörper (N-Term) (ABIN6991979)

Target: ME1 (Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ME1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-ME1 Antikörper

Bindungsspezifität: N-Term

Spezifität: ME1 antibody is human, mouse and rat reactive. ME1 antibody is predicted not to cross-react with ME2.

Aufreinigung: ME1 antibody is affinity chromatography purified via peptide column.

Immunogen: ME1 antibody was raised against a 16 amino acid peptide near the amino terminus of human ME1. The immunogen is located within the first 50 amino acids of ME1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ME1 antibody can be used for detection of ME1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry at 5 μ,g/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: ME1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: ME1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Details zu ME1

Target: ME1 (Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1))

Andere Bezeichnung: ME1

Hintergrund: ME1, also known as NADP-ME, MES or HUMNDME, may play an important role as a housekeeping protein within the cell (1). ME1 is a 572 amino acid cytoplasmic protein that belongs to the malic enzyme family (2). It is expressed ubiquitously with highest expression in liver and white adipose tissue. ME1 functions as an NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis (3). The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles (3,4). ME1 is regulated by both thyroid hormone levels and the amount of carbohydrates in the diet (5).

Molekulargewicht: Predicted: 63 kDa

Observed: 63 kDa

Gen-ID: 4199

NCBI Accession: NP_002386

UniProt: P48163

Pathways: Regulation of Lipid Metabolism by PPARalpha