ELISA, Western Blotting (WB), Immunofluorescence (IF)
Spezifität
At least three isoforms of SIPA1 are known to exist, this antibody will recognize all three isoforms. This antibody is specific for SIPA1 and will not recognize the other SIPA1 family of proteins.
Aufreinigung
SIPA1 Antibody is affinity chromatography purified via peptide column.
Immunogen
SIPA1 antibody was raised against a 19 amino acid synthetic peptide from near the amino terminus of human SIPA1. The immunogen is located within amino acids 40 - 90 of SIPA1.
SIPA1
Reaktivität: Human
WB, IF, EIA
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
SIPA1 antibody can be used for detection of EPAC1 by Western blot at 1 μ,g/mL. Antibody can also be used for immunofluorescence starting at 20 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1 mg/mL
Buffer
SIPA1 Antibody is supplied in PBS containing 0.02 % sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C,4 °C
Informationen zur Lagerung
SIPA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
SIPA1 Antibody: Signal-induced proliferation 1 (SIPA1) is a member of the SIPA1 family of RapGAPs, a family of mitogen-induced GTPase activating proteins. SIPA1 exhibits a specific GAP activity for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases. When abnormally or prematurely expressed, SIPA1 may also hamper mitogen-induced cell cycle progression and may increase metastases in human cancers.