ZNF219 Antikörper (N-Term)

Produktnummer ABIN6991505

Der Kaninchen Polyklonal Anti-ZNF219-Antikörper wurde für WB, ELISA, IHC (p) und IF validiert. Er ist geeignet, ZNF219 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für ZNF219 Antikörper (N-Term) (ABIN6991505)

Target: ZNF219 (Zinc Finger Protein 219 (ZNF219))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ZNF219 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Produktdetails anti-ZNF219 Antikörper

Bindungsspezifität: AA 90-140, N-Term

Spezifität: At least two isoforms of ZFP219 are known to exist, this antibody will recognize both.

Aufreinigung: ZFP219 Antibody is affinity chromatography purified via peptide column.

Immunogen: ZFP219 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human ZFP219. The immunogen is located within amino acids 90 - 140 of ZFP219.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ZFP219 antibody can be used for detection of ZFP219 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: ZFP219 Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: ZFP219 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu ZNF219

Target: ZNF219 (Zinc Finger Protein 219 (ZNF219))

Andere Bezeichnung: ZFP219

Hintergrund: ZFP219 Antibody: ZFP219 is a developmentally regulated member of the Kruppel-like zinc finger gene family that is thought to function as a transcriptional repressor. Yeast two-hybrid screening showed association with Sox9, a transcription factor that is essential for chondrogenesis. ZFP219 is specifically expressed in the developing limb buds and colocalizes with Sox9 in the nucleus. Knockdown of ZFP219 expression decreased Sox9-induced mRNA expression, and a dominant-negative mutant of ZFP219 inhibited Bmp2-induced chondrocyte differentiation, suggesting that ZFP219 plays an important role as a transcriptional partner of Sox9 in the regulation of chondrocyte differentiation.

Molekulargewicht: Predicted: 80 kDa

Observed: 78 kDa

Gen-ID: 51222

NCBI Accession: NP_001095142

UniProt: Q9P2Y4