GOLPH3 Antikörper (N-Term)

Produktnummer ABIN6991206

Dieses Anti-GOLPH3-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von GOLPH3 in WB, ELISA und IF. Geeignet für Human, Maus und Ratte.

Kurzübersicht für GOLPH3 Antikörper (N-Term) (ABIN6991206)

Target: GOLPH3 (Golgi phosphoprotein 3 (Coat-Protein) (GOLPH3))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GOLPH3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Produktdetails anti-GOLPH3 Antikörper

Bindungsspezifität: N-Term

Aufreinigung: GOLPH3 Antibody is affinity chromatography purified via peptide column.

Immunogen: GOLPH3 antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human GOLPH3. The immunogen is located within the first 50 amino acids of GOLPH3.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: GOLPH3 antibody can be used for detection of GOLPH3 by Western blot at 0.5 - 1 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: GOLPH3 Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: GOLPH3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu GOLPH3

Target: GOLPH3 (Golgi phosphoprotein 3 (Coat-Protein) (GOLPH3))

Andere Bezeichnung: GOLPH3

Hintergrund: GOLPH3 Antibody: GOLPH3 was initially identified as a peripheral membrane protein localized to the trans-Golgi network, but others reported it to be primarily a mitochondrial protein that regulated the mitochondrial mass through the regulation of the mitochondria-specific phospholipid cardiolipin. GOLPH3 has since been implicated in the target of rapamycin (TOR) signalling pathway. Its overexpression in transfected cells led to and increase in anchorage-independent growth and cell proliferation in vitro. Furthermore, GOLPH3-transfected cells enhanced S6 Kinase activity in response to growth factor stimulation by EGF. Simultaneously, AKT phosphorylation increased in these cells, while these events were abrogated in GOLPH3 siRNA treated cells compared to control cells, indicating the GOLPH3 can enhance signalling through TOR-associated complexes. These results suggest that GOLPH3 is a bona fide oncogene and may be a useful target for therapeutic strategies.

Gen-ID: 64083

UniProt: Q9H4A6