THEM4 Antikörper (AA 60-110)

Produktnummer ABIN6991139

Der Kaninchen Polyklonal anti-THEM4 Antikörper wird verwendet zum Nachweis von THEM4 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, ELISA, IF und IHC (p).

Kurzübersicht für THEM4 Antikörper (AA 60-110) (ABIN6991139)

Target: THEM4 (Thioesterase Superfamily Member 4 (THEM4))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser THEM4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-THEM4 Antikörper

Bindungsspezifität: AA 60-110

Spezifität: The additional higher molecular weight bands seen in the immunoblot may represent post-translationally modified TMEM4.

Aufreinigung: THEM4 Antibody is affinity chromatography purified via peptide column.

Immunogen: THEM4 antibody was raised against an 18 amino acid synthetic peptide near the center of human THEM4. The immunogen is located within amino acids 60 - 110 of THEM4.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: THEM4 antibody can be used for detection of THEM4 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: THEM4 Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: THEM4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu THEM4

Target: THEM4 (Thioesterase Superfamily Member 4 (THEM4))

Andere Bezeichnung: THEM4

Hintergrund: THEM4 Antibody: THEM4, also known as CTMP, binds specifically to the carboxy-terminal regulatory domain of PKB/Akt at the plasma membrane and acts as a negative regulator, reversing the phenotype of v-Akt-transformed cells. Hypermethylation of the THEM4 promoter and transcriptional downregulation of the gene has been reported in multiple glioblastomas, suggesting that epigenetic regulation of THEM4 may play a role in the progression of this cancer. Bioinformatic analysis, confirmed by in vitro testing, indicates that THEM4 is a broad-range, high activity acyl-CoA thioesterase. Recent reports have also indicated that TMEM4 is a mitochondrial protein whose overexpression is associated with an increase in mitochondrial membrane depolarization and caspase-3 and PARP cleavage, suggesting that THEM4 is involved in the apoptotic program.

Gen-ID: 117145

NCBI Accession: NP_444283

UniProt: Q5T1C6

Pathways: Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung