Occludin Antikörper (C-Term)

Produktnummer ABIN6991125

Dieses Anti-Occludin-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Occludin in WB, ELISA, IF und IHC (p). Geeignet für Human, Maus und Ratte.

Kurzübersicht für Occludin Antikörper (C-Term) (ABIN6991125)

Target: Occludin (OCLN)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Occludin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Occludin Antikörper

Bindungsspezifität: C-Term

Spezifität: At least three isoforms of OCLN are known to exist.

Aufreinigung: OCLN Antibody is affinity chromatography purified via peptide column.

Immunogen: OCLN antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human OCLN. The immunogen is located within the last 50 amino acids of OCLN.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: OCLN antibody can be used for detection of OCLN by Western blot at 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: OCLN Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: OCLN antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu Occludin

Target: Occludin (OCLN)

Andere Bezeichnung: OCLN

Hintergrund: OCLN Antibody: Tight junctions act as a semi-permeable barrier to the transport of ions, solutes, and water and are considered to function as a fence that divides apical and basolateral domains of plasma membranes. Tight junctions coordinate a variety of signaling and trafficking molecules regulating cell differentiation, proliferation, and polarity and contain a number of junctional proteins including Occludin, Claudins, junctional adhesion molecules (JAMs), as well as multiple scaffold proteins. Occludin, the first identified component of tight junction strands, is thought function as a signal transmitter in multiple signaling pathways and can associate with multiple kinases and phosphatases such as PI3-kinase and protein phosphatases 1 and 2A. At least two isoforms of OCLN are known to exist.

Molekulargewicht: Predicted: 57 kDa

Observed: 57 kDa

Gen-ID: 4950

UniProt: Q16625

Pathways: Cell-Cell Junction Organization, Hepatitis C