EBI3 Antikörper (AA 150-200)

Produktnummer ABIN6991042

Dieser Kaninchen Polyklonal Antikörper erkennt spezifisch EBI3 in WB, ELISA, IHC (p) und IF. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für EBI3 Antikörper (AA 150-200) (ABIN6991042)

Target: EBI3 (IL-27b) (Interleukin-27 subunit beta (IL-27b))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EBI3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Produktdetails anti-EBI3 Antikörper

Bindungsspezifität: AA 150-200

Aufreinigung: EBI3 Antibody is affinity chromatography purified via peptide column.

Immunogen: Anti-EBI-3 antibody was raised against a peptide corresponding to 16 amino acids near the center of mature human EBI-3. The immunogen is located within amino acids 150 - 200 of EBI3.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 0.5 - 2 μ,g/mL, IF: 20 μ,g/mL , IHC: 2.5 μ,g/mL,.

Antibody validated: Western Blot in human and rat samples, Immunofluorescence and Immunohistochemistry in human samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: EBI3 Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: EBI3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu EBI3

Target: EBI3 (IL-27b) (Interleukin-27 subunit beta (IL-27b))

Andere Bezeichnung: EBI3

Hintergrund: EBI3 Antibody: EBI3 is a subunit in two distinct heterodimeric cytokines: interleukin-27 (IL-27) and IL-35. Like interleukin-23 (IL-23), IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the p40-related protein EBI3 and an IL-12 p35-related protein termed p28. IL-27 is produced after activation by antigen-presenting cells and induces proliferation of naive but not memory CD4+ T-cells. It acts by binding to its receptor WSX-1 (also known as TCCR) and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting the IL-27 is involved in the regulation of immune processes. It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-gamma, and T-bet. IL-35 is composed of EBI3 and the p35 subunit of IL-12 and has been reported to have therapeutic effects against collagen-induced arthritis by expanding the population of regulatory T cells and suppressing Th17 cells. At least two isoform of EBI3 are known to exist.

Molekulargewicht: Predicted: 25kD

Observed: 30 kD (25kD + 2 N-Glycosylations) kDa

Gen-ID: 10148

NCBI Accession: NP_005746

UniProt: Q14213