Syntaphilin Antikörper (N-Term)

Produktnummer ABIN6990911

Der Kaninchen Polyklonal Anti-Syntaphilin-Antikörper wurde für WB, ELISA, IHC (p) und IF validiert. Er ist geeignet, Syntaphilin in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für Syntaphilin Antikörper (N-Term) (ABIN6990911)

Target: Syntaphilin (SNPH)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Syntaphilin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Produktdetails anti-Syntaphilin Antikörper

Bindungsspezifität: AA 80-130, N-Term

Aufreinigung: Syntaphilin Antibody is affinity chromatography purified via peptide column.

Immunogen: Syntaphilin antibody was raised against a 18 amino acid synthetic peptide from near the amino terminus of human Syntaphilin. The immunogen is located within amino acids 80 - 130 of Syntaphilin.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Syntaphilin antibody can be used for detection of Syntaphilin by Western blot at 2 - 4 μ,g/mL. Despite its predicted molecular weight, Syntaphilin usually migrates at higher molecular weight in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Syntaphilin Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Syntaphilin antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu Syntaphilin

Target: Syntaphilin (SNPH)

Andere Bezeichnung: Syntaphilin

Hintergrund: Syntaphilin Antibody: Syntaphilin was initially identified in a yeast two-hybrid screen with the carboxy terminal region of Syntaxin-1 as bait. Syntaxin-1 is a key component of the synaptic vesicle docking machinery that forms the SNARE complex with synaptobrevin and SNAP-25. Syntaphilin competes with SNAP-25 for binding to syntaxin-1 and inhibits the formation of the SNARE complex, thereby potentially regulating synaptic vesicle exocytosis. Syntaphilin also binds dynamin-1 and inhibits dynamin-dependent endocytosis. Mice lacking syntaphilin show an increased level of mitochondrial motility and a reduced density of axonal mitochondria. This correlates with an enhanced short-term facilitation and significant impairments in motor ability, suggesting syntaphilin plays a major role in presynaptic function. Multiple isoforms are known to exist.

Gen-ID: 9751

UniProt: O15079

Pathways: Synaptic Vesicle Exocytosis