IRGM Antibody is affinity chromatography purified via peptide column.
Immunogen
IRGM antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of the human IRGM. The immunogen is located within amino acids 30 - 80 of IRGM.
IRGM
Reaktivität: Human
WB, IF (cc), IF (p)
Wirt: Kaninchen
Polyclonal
Alexa Fluor 680
Applikationshinweise
IRGM antibody can be used for detection of IRGM by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1 mg/mL
Buffer
IRGM Antibody is supplied in PBS containing 0.02 % sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C,4 °C
Informationen zur Lagerung
IRGM antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ifi1 antikoerper, Iigp3 antikoerper, Iipg3 antikoerper, Irgm antikoerper, LRG-47 antikoerper, IFI1 antikoerper, IRGM1 antikoerper, LRG47 antikoerper, immunity-related GTPase family M member 1 antikoerper, immunity-related GTPase M antikoerper, immunity related GTPase M antikoerper, immunity-related GTPase family M protein antikoerper, Irgm1 antikoerper, Irgm antikoerper, IRGM antikoerper, LOC481473 antikoerper
Hintergrund
IRGM Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. Two of the strongest hits implicate genes IRGM and ATG16L1, which encode proteins thought to be critical to the autophagy pathway and being significantly associated with Crohn's disease. IRGM induces autophagy and generates large autolysosomal organelles as a mechanism for the elimination of intracellular Mycobacterium tuberculosis. In mouse, IRGM belongs to a family of gamma-interferon-induced GTP-binding proteins of approximately 48 kDa that also includes IRGM2 and IRGM3, this antibody may also recognize these proteins.