BRCC3 Antikörper (N-Term)

Produktnummer ABIN6990795

Dieses Anti-BRCC3-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von BRCC3 in WB, ELISA, IHC (p) und IF. Geeignet für Human, Maus und Ratte.

Kurzübersicht für BRCC3 Antikörper (N-Term) (ABIN6990795)

Target: BRCC3 (BRCA1/BRCA2-Containing Complex, Subunit 3 (BRCC3))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BRCC3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Produktdetails anti-BRCC3 Antikörper

Bindungsspezifität: AA 20-70, N-Term

Aufreinigung: BRCC36 Antibody is affinity chromatography purified via peptide column.

Immunogen: BRCC36 antibody was raised against a 18 amino acid synthetic peptide from near the amino terminus of human BRCC36. The immunogen is located within amino acids 20 - 70 of BRCC36.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: BRCC36 antibody can be used for detection of BRCC36 by Western blot at 0.5 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: BRCC36 Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: BRCC36 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu BRCC3

Target: BRCC3 (BRCA1/BRCA2-Containing Complex, Subunit 3 (BRCC3))

Andere Bezeichnung: BRCC36

Hintergrund: BRCC36 Antibody: Together with the breast and ovarian predisposition proteins BRCA1 and BRCA2 and RAD51 and BRCC45, BRCC36 forms a holoenzyme complex that possesses a ubiquitin E3 ligase activity. Aberrant levels of BRCC36 were detected in sporadic breast tumors and depletion of either BRCC36 or BRCC45 by siRNA resulted in increased sensitivity to ionizing radiation and defects in the G2/M checkpoint, indicating that BRCC36 acts to enhance cellular survival following DNA damage. Recent experiments have shown that BRCC36 is essential for ionizing radiation-induced BRCA1 phosphorylation and nuclear foci formation, suggesting that BRCC36 may be an important target in the treatment of radiation-resistant breast tumors. At least two isoforms of BRCC36 are known to exist.

Molekulargewicht: Predicted: 35 kDa

Observed: 35 kDa

Gen-ID: 79184

NCBI Accession: NP_077308

UniProt: P46736

Pathways: Positive Regulation of Response to DNA Damage Stimulus