TRIM25 Antikörper (C-Term)

Produktnummer ABIN6990789

Dieses Anti-TRIM25-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von TRIM25 in WB, ELISA, IF und ICC. Geeignet für Human und Maus.

Kurzübersicht für TRIM25 Antikörper (C-Term) (ABIN6990789)

Target: TRIM25 (Tripartite Motif Containing 25 (TRIM25))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TRIM25 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-TRIM25 Antikörper

Bindungsspezifität: AA 520-570, C-Term

Aufreinigung: TRIM25 Antibody is affinity chromatography purified via peptide column.

Immunogen: TRIM25 antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human TRIM25. The immunogen is located within amino acids 520 - 570 of TRIM25.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: TRIM25 antibody can be used for detection of TRIM25 by Western blot at 1 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 20 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: TRIM25 Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: TRIM25 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu TRIM25

Target: TRIM25 (Tripartite Motif Containing 25 (TRIM25))

Andere Bezeichnung: TRIM25

Hintergrund: TRIM25 Antibody: TRIM25, also known as estrogen responsive finger protein (EFP) is a member of the RING finger-B box-coiled coil family and is a downstream target of estrogen receptor a. Mice lacking this gene displayed an underdeveloped uterus and reduced estrogen responsiveness demonstrating that this protein is essential for estrogen dependent proliferation. TRIM25 expression is increased in breast carcinoma where it promotes the growth of breast tumor by functioning as an E3 ubiquitin ligase that targets the negative cell cycle checkpoint 14-3-3s. Recent reports have indicated that TRIM25 E3 ubiquitin ligase is essential for the retinoic-acid-inducible gene 1 (RIG-1) signaling pathway to elicit host antiviral innate immunity.

Gen-ID: 7706

NCBI Accession: NP_005073

UniProt: Q14258