KEAP1 Antikörper (C-Term)

Produktnummer ABIN6990770

Der Kaninchen Polyklonal Anti-KEAP1-Antikörper wurde für WB, ELISA und IF validiert. Er ist geeignet, KEAP1 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für KEAP1 Antikörper (C-Term) (ABIN6990770)

Target: KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser KEAP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Produktdetails anti-KEAP1 Antikörper

Bindungsspezifität: C-Term

Spezifität: At least two isoforms of KEAP1 are known to exist.

Aufreinigung: KEAP1 Antibody is affinity chromatography purified via peptide column.

Immunogen: Anti-KEAP1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human KEAP1. The immunogen is located within the last 50 amino acids of KEAP1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1-2 μ,g/mL, IF: 20 μ,g/mL.

Antibody validated: Western Blot in human, mouse and rat samples, Immunofluorescence in human samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: KEAP1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: KEAP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu KEAP1

Target: KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))

Andere Bezeichnung: KEAP1

Hintergrund: KEAP1 Antibody: KEAP1 (kelch-like ECH-associated protein 1) is a stress sensing adaptor for the Cullin3 (Cul3)-dependent E3 ubiquitin ligase complex that negatively regulates NRF2 (NF-E2-related factor 2) and plays a role in the oxidative stress response. It targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome. KEAP1 contains an amino terminal BTB/POZ domain and a carboxyl terminal KELCH domain which are required for interaction with NRF2, and in binding Cul3-E3 ubiquitin ligase. Altered expression of NRF2 is associated with chronic obstructive pulmonary disease (COPD). KEAP1 also targets the down regulation of NF-κ,B activity by targeting IKKβ, degradation. Mutation of the KEAP1 gene is found in lung cancer.

Molekulargewicht: Predicted: 70kD

Observed: 65-70kD kDa

Gen-ID: 9817

NCBI Accession: NP_987096

UniProt: Q14145

Pathways: Maintenance of Protein Location