Reticulon 4 Antikörper (N-Term)

Produktnummer ABIN6990735

Produktdetails anti-Reticulon 4 Antikörper

Target: Reticulon 4 (RTN4)

Bindungsspezifität: N-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Reticulon 4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Spezifität: At least five isoforms of Nogo are known to exist, this antibody is specific for NogoA.

Aufreinigung: NogoA Antibody is affinity chromatography purified via peptide column.

Immunogen: NogoA antibody was raised against a 23 amino acid synthetic peptide from near the amino terminus of human NogoA. The immunogen is located within the first 50 amino acids of NogoA.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: NogoA antibody can be used for detection of NogoA by Western blot at 0.5 - 1 μ,g/mL. Despite its predicted molecular weight, NogoA typically migrates at 180 kDa in an SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: NogoA Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: NogoA antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu Reticulon 4

Target: Reticulon 4 (RTN4)

Andere Bezeichnung: NogoA (RTN4 Produkte)

Hintergrund: NogoA Antibody: NogoA is a member of a family of integral membrane proteins termed reticulons that are thought to be involved in numerous disorders including neurodegenerative diseases. Reticulon proteins are known to regulate many cellular processes and interact with multiple proteins and receptors such as BACE. NogoA was initially identified as a myelin-associated neurite outgrowth inhibitor. It is highly expressed in oligodendrocytes in the white matter of the CNS, blocking its activity with antibodies or other factors results in improved axon regrowth and functional recovery in experimental CNS lesion models. NogoA has also been suggested to play a role in neurodegenerative diseases such as Amyotrophic lateral sclerosis, in which case NogoA is found at elevated levels in postmortem muscular samples, and multiple sclerosis (MS), in which case autoantibodies to NogoA have been found in serum and cerebrospinal fluid in MS patients.

Gen-ID: 57142

NCBI Accession: NP_065393

UniProt: Q9NQC3

Pathways: Neurotrophin Signalübertragung, Regulation of Cell Size, SARS-CoV-2 Protein Interaktom