PTRH2 Antikörper (AA 80-130)

Produktnummer ABIN6990570

Dieses Anti-PTRH2-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von PTRH2 in WB, ELISA, IF und IHC (p). Geeignet für Human und Maus.

Kurzübersicht für PTRH2 Antikörper (AA 80-130) (ABIN6990570)

Target: PTRH2 (Peptidyl-tRNA Hydrolase 2 (PTRH2))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PTRH2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-PTRH2 Antikörper

Bindungsspezifität: AA 80-130

Aufreinigung: Bit1 Antibody is affinity chromatography purified via peptide column.

Immunogen: Bit1 antibody was raised against a 16 amino acid synthetic peptide from near the center of human Bit1. The immunogen is located within amino acids 80 - 130 of Bit1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Bit1 antibody can be used for the detection of Bit1 by Western blot at 1 - 4 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 10 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Bit1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Bit1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu PTRH2

Target: PTRH2 (Peptidyl-tRNA Hydrolase 2 (PTRH2))

Andere Bezeichnung: Bit1

Hintergrund: Bit1 Antibody: Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. Bit1 (Bcl-2 inhibitor of transcription 1) was recently identified as being involved in this process. Bit1 is a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis where it forms a complex with AES, a small Groucho/transducin-like enhancer of split (TLE) protein and induces caspase-independent apoptosis. Both AES and TLE proteins are transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. It has been suggested that Bit1-AES complexes turn off a survival-promoting gene transcription program controlled by TLE. Interestingly, apoptosis of cells transfected with Bit1 and AES could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin.

Gen-ID: 51651

NCBI Accession: NP_057161

UniProt: Q9Y3E5