XAF1 Antikörper (AA 230-280)

Produktnummer ABIN6990495

Dieses Anti-XAF1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von XAF1 in WB, ELISA und IF. Geeignet für Human.

Kurzübersicht für XAF1 Antikörper (AA 230-280) (ABIN6990495)

Target: XAF1 (XiAP Associated Factor 1 (XAF1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser XAF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Produktdetails anti-XAF1 Antikörper

Bindungsspezifität: AA 230-280

Aufreinigung: XAF-1 Antibody is affinity chromatography purified via peptide column.

Immunogen: XAF-1 antibody was raised against a synthetic peptide corresponding to 16 amino acids near the middle of human XAF-1. The immunogen is located within amino acids 230 - 280 of XAF-1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: XAF-1 antibody can be used for the detection of XAF-1 by Western blot at 0.5 - 1 μ,g/mL. Multiple isoforms of XAF-1 exist and a doublet at ~30 kDa can be seen using anti-XAF-1 (IN). For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: XAF-1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: XAF-1antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu XAF1

Target: XAF1 (XiAP Associated Factor 1 (XAF1))

Andere Bezeichnung: XAF-1

Hintergrund: XAF-1 Antibody: XAF-1 binds to XIAP, an inhibitor of caspases-3, -7, and -9, and triggers its relocation from the cytosol to the nucleus. Overexpression of XAF-1 results in the neutralization of XIAP's ability to inhibit cell death. XAF-1 is normally expressed in all adult and fetal tissues but was found to be present in very low levels in a variety of cancer cell lines. In contrast, XIAP levels have been shown to be high in a majority of cell lines. Low XAF-1 and high basal expression of XIAP may therefore play a critical role in maintaining survival of cancer cell lines. Both IFN-alpha2 and IFN-beta can induce XAF-1 mRNA in all cells examined but induction of XAF-1 protein (as observed by immunoblot analysis) was seen only in cell lines sensitive to the apoptotic effects of IFNs.

Molekulargewicht: Predicted: 31, 33 kDa

Observed: 31 kDa

Gen-ID: 54739

UniProt: Q6GPH4