PIKE (C-Term) Antikörper

Produktnummer ABIN6990467

Produktdetails

Target: PIKE

Bindungsspezifität: C-Term

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: Anti-PIKE will detect both PIKE-L and PIKE-A isoforms.

Aufreinigung: PIKE Antibody is Ion exchange chromatography purified.

Immunogen: PIKE antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of human PIKE. The immunogen is located within the last 50 amino acids of PIKE.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: PIKE antibody can be used for detection of PIKE by Western blot at 0.5 to 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 10 μ,g/mL.

Antibody validated: Western Blot in mouse samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PIKE Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: PIKE antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Antigendetails

Target: PIKE

Synonyme: CENTG1 antikoerper, GGAP2 antikoerper, PIKE antikoerper, ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 antikoerper, AGAP2 antikoerper

Hintergrund: PIKE Antibody: Phosphoinositide 3 kinase enhancer (PIKE) is a recently identified nuclear GTPase that interacts with nuclear phosphoinositide 3-kinase (PI3 kinase) to stimulate its lipid kinase activity. PIKE exists in multiple isoforms, a shorter C-terminal isoform (PIKE-A) has also been identified as centaurin gamma 1. The longest isoform (PIKE-L) has been shown to bind to the adaptor protein Homer and thereby link to metabotropic glutamate receptors, leading to activation of PI3 kinase activity and prevention of neuronal apoptosis. Overexpression of PIKE-A enhances Akt activity and promotes cancer cell invasion, whereas decreased expression of PIKE-A via dominant negative expression of PIKE-A or PIKE-A knockdown inhibits these processes. In many human cancers, expression of PIKE-A is enhanced, leading to increased Akt activity and preventing apoptosis.

Gen-ID: 116986

UniProt: Q99490