XAF1 Antikörper (C-Term)

Produktnummer ABIN6990444

Produktdetails anti-XAF1 Antikörper

Target: XAF1 (XiAP Associated Factor 1 (XAF1))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser XAF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Aufreinigung: XAF-1 Antibody is affinity chromatography purified via peptide column.

Immunogen: XAF-1 antibody was raised against a synthetic peptide corresponding to amino acids at the C-terminus of human XAF-1. The immunogen is located within the last 50 amino acids of XAF-1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: XAF-1 antibody can be used for the detection of XAF-1 by Western blot at 0.5 to 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2 μ,g/mL. For immunofluorescence start at 10 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: XAF-1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: XAF-1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu XAF1

Target: XAF1 (XiAP Associated Factor 1 (XAF1))

Andere Bezeichnung: XAF-1 (XAF1 Produkte)

Synonyme: BIRC4BP antikoerper, HSXIAPAF1 antikoerper, XIAPAF1 antikoerper, Fbxo39 antikoerper, XIAP associated factor 1 antikoerper, xaf1 antikoerper, XAF1 antikoerper, Xaf1 antikoerper

Hintergrund: XAF-1 Antibody: XAF-1 binds to XIAP, an inhibitor of caspases-3, -7, and -9, and triggers its relocation from the cytosol to the nucleus. Overexpression of XAF-1 results in the neutralization of XIAP's ability to inhibit cell death. XAF-1 is normally expressed in all adult and fetal tissues but was found to be present in very low levels in a variety of cancer cell lines. In contrast, XIAP levels have been shown to be high in a majority of cell lines. Low XAF-1 and high basal expression of XIAP may therefore play a critical role in maintaining survival of cancer cell lines. Both IFN-alpha2 and IFN-beta can induce XAF-1 mRNA in all cells examined but induction of XAF-1 protein (as observed by immunoblot analysis) was seen only in cell lines sensitive to the apoptotic effects of IFNs.

Molekulargewicht: Predicted: 35 kDa

Observed: 32 kDa

Gen-ID: 54739

UniProt: Q6GPH4