CXCR4 Antikörper (N-Term)

Produktnummer ABIN6990364

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch CXCR4 in WB, ELISA, FACS, IF, IP, IHC (p) und ICC. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für CXCR4 Antikörper (N-Term) (ABIN6990364)

Target: CXCR4 (Chemokine (C-X-C Motif) Receptor 4 (CXCR4))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CXCR4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Produktdetails anti-CXCR4 Antikörper

Bindungsspezifität: N-Term

Spezifität: CXCR4 Antibody is predicted to not cross-react with other CXCR familiy members.

Aufreinigung: CXCR4 Antibody is affinity chromatography purified via peptide column.

Immunogen: Anti-CXCR4 antibody was raised against a peptide corresponding to 14 amino acids near the amino terminus of human CXCR4 isoform b. The immunogen is located within the first 50 amino acids of CXCR4.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1 - 2 μ,g/mL, IP/ ICC: 2 μ,g/mL, IHC-P: 5 μ,g/mL, IF: 20 μ,g/mL, Flow Cyt: 0.1 μ,g/mL.

Antibody validated: Western Blot in human, mouse, and rat samples, Immunohistochemistry, Immunocytochemistry and Immunofluorescence in human samples, Flow Cytometry in human and mouse samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: CXCR4 Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: CXCR4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu CXCR4

Target: CXCR4 (Chemokine (C-X-C Motif) Receptor 4 (CXCR4))

Andere Bezeichnung: CXCR4

Hintergrund:

CXCR4, a G-protein coupled receptor (GPCR) with seven transmembrane domains, is a CXC chemokine receptor specific for stromal-derived-factor-1 (SDF-1 or CXCL12). CXCR4 was initially discovered as one of the co-receptors for HIV entry into CD4+ T cells (1). Blocking CXCR4 could be potentially used as novel therapeutics for HIV treatment.

CXCR4 signaling plays an important role in the migration, proliferation and quiescence of hematopoietic stem cell and their retention within the bone marrow, where it has high levels of SDF-1/CXCL12(2). It has been demonstrated that CXCR4 signaling mediates CD20 up-regulation on B cells (3).

CXCR4 is highly expressed in more than 23 types of cancer, including breast cancer, ovarian cancer, melanoma, and prostate cancer, while there is very less or no expression of CXCR4 in healthy tissues. CXCR4 expression in cancer cells has been reported to be associated with tumor survival, growth and metastasis in tissues with high levels of SDF-1/CXCL12, such as lungs, liver and bone marrow (4,5).

CXCR4 has been shown to regulate neuronal migration, cell positioning and axon wiring (6,7). CXCR4 mutant mice displayed aberrant neuronal distribution, which implicates the role in neuronal disorders such as epilepsy. CXCR4 is also involved in WHIM syndrome (8). WHIM mutations in CXCR4 were recently found in patients with Waldenstrom's macroglobulinemia, and these mutations are correlated to clinical resistance to ibrutinib (9,10).

Molekulargewicht: Predicted: 40 kDa

Observed: 44 kDa (Post-modification: 2 N-linked glycosylation)

Gen-ID: 7852

NCBI Accession: NP_003458

UniProt: P61073

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events