IKAP/p150 Antikörper (C-Term)

Produktnummer ABIN6990353

Produktdetails anti-IKAP/p150 Antikörper

Target: IKAP/p150 (ELP1) (Elongator Complex Protein 1 (ELP1))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IKAP/p150 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Spezifität: At least two isoforms of IKAP are known two exist, this antibody will detect both isoforms.

Aufreinigung: IKAP Antibody is affinity chromatography purified via peptide column.

Immunogen: IKAP antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human IKAP. The immunogen is located within the last 50 amino acids of IKAP.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IKAP antibody can be used for detection of IKAP by Western blot at 0.5 to 1 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 1 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: IKAP Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: IKAP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu IKAP/p150

Target: IKAP/p150 (ELP1) (Elongator Complex Protein 1 (ELP1))

Andere Bezeichnung: IKAP (ELP1 Produkte)

Synonyme: DYS antikoerper, ELP1 antikoerper, FD antikoerper, IKAP antikoerper, IKI3 antikoerper, TOT1 antikoerper, IKBKAP antikoerper, DKFZp469N1415 antikoerper, 3110040G09Rik antikoerper, 6030413P05 antikoerper, C78473 antikoerper, Elp1 antikoerper, Ikbkap antikoerper, elongator complex protein 1 antikoerper, inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase complex-associated protein antikoerper, ielongator complex protein 1 S homeolog antikoerper, inhibitor of kappa light polypeptide enhancer in B cells, kinase complex-associated protein antikoerper, ELP1 antikoerper, Elp1 antikoerper, IKBKAP antikoerper, elp1.S antikoerper, elp1 antikoerper, Ikbkap antikoerper

Hintergrund: IKAP Antibody: IKAP was initially identified as a scaffold protein of the Iκ,B kinase complex that could bind to IKKα, IKKβ, NF-κ,B, and the NF-κ,B-inducing kinase (NIK), although later evidence has cast doubt on this. More recent reports show that mutations in IKAP such as a frameshift leading to a truncated protein or a missense mutation that leads to defective phosphorylation are responsible for the autosomal recessive genetic disease familial dysautonomia (FD). Reports indicating that it forms part of the RNA polymerase II transcription elongation complex suggest that this disease may be due to compromised transcription elongation. More recently, it was shown that IKAP associates with c-Jun N-terminal kinase (JNK) and could specifically enhance JNK activation induced by the upstream JNK activators MEKK1 and ASK1, indicating another possible cause for FD.

Molekulargewicht: Predicted: 134, 147 kDa

Observed: 105, 145 kDa

Gen-ID: 8518

UniProt: O95163