GSK3 alpha/beta Antikörper (pTyr216, pTyr279)

Produktnummer ABIN6989926

Dieser Kaninchen Monoklonal Antikörper detektiert spezifisch GSK3 alpha/beta in WB, IHC, IHC (p), IF (cc) und IP. Es zeigt Reaktivität gegenüber Proben von Human, Ratte und Maus.

Kurzübersicht für GSK3 alpha/beta Antikörper (pTyr216, pTyr279) (ABIN6989926)

Target: GSK3 alpha/beta (GSK3a/b) (Glycogen Synthase Kinase 3 alpha/beta (GSK3a/b))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser GSK3 alpha/beta Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunoprecipitation (IP)

Klon: 5G8

Produktdetails anti-GSK3 alpha/beta Antikörper

Bindungsspezifität: pTyr216, pTyr279

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Purified by Protein A.

Immunogen: Synthetic phospho-Peptide corresponding to residues surrounding Tyr216 and 279 of human GSK3(alpha+beta).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
IHC-P 1:200-400
IF(ICC) 1:50-200
IP(1-2 μg)
IHC()

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu GSK3 alpha/beta

Target: GSK3 alpha/beta (GSK3a/b) (Glycogen Synthase Kinase 3 alpha/beta (GSK3a/b))

Andere Bezeichnung: GSK3(alpha+beta)

Hintergrund:

Synonyms: Factor A antibody, Glycogen synthase kinase 3 alpha antibody, Glycogen synthase kinase 3 beta antibody, GSK3 alpha antibody, GSK3 beta antibody, GSK3B antibody

Background: Glycogen synthase kinase 3 (GSK3) is a proline directed serine threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3s substrate specificity is unique in that phosphorylation of substrate only occurs if a phosphoserine or phosphotyrosine is present four residues C terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, alpha and beta, and they show a high degree of amino acid homology. The two isoforms of GSK3 are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3 alpha) by protein kinase B (PKB) causes its inactivation is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor 1 (HSF1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimer's disease. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.

Gen-ID: 2932

UniProt: P49840