TAP Tag Antikörper (HRP)

Produktnummer ABIN6987469

Produktdetails

Target: TAP Tag

Reaktivität: Tag

Wirt: Maus

Klonalität: Monoklonal

Konjugat: HRP

Applikation: Western Blotting (WB), ELISA

Kreuzreaktivität: Tag

Kreuzreaktivität (Details): TAP-Tag

Aufreinigung: Purified by Protein A.

Immunogen: Recombinant TAP-Tag

Klon: Mix-mA

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: TAP Tag

Andere Bezeichnung: TAP-Tag

Substanzklasse: Tag

Hintergrund:

Synonyms: TAP Tag, TAPTag, Tandem affinity purification

Background: Tandem affinity purification (TAP) is a purification technique for studying proteinprotein interactions. It involves creating a fusion protein with a designed piece, the TAP tag, on the end. The original TAP method involves the fusion of the TAP tag to the C-terminus of the protein under study. The TAP tag consists of calmodulin binding peptide (CBP) from the N-terminal, followed by tobacco etch virus protease (TEV protease) cleavage site and Protein A, which binds tightly to IgG. The relative order of the modules of the tag is important because Protein A needs to be at the extreme end of the fusion protein so that the entire complex can be retrieved using an IgG matrix.