p300 Antikörper

Produktnummer ABIN6972466

Dieses Anti-p300-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von p300 in WB, IP, ICC, IF, ChIP und ChIP-seq. Geeignet für Human.

Kurzübersicht für p300 Antikörper (ABIN6972466)

Target: p300 (EP300) (E1A Binding Protein P300 (EP300))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser p300 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq)

Klon: NM11

Produktdetails anti-p300 Antikörper

Produktmerkmale: p300 (E1A binding protein p300) functions as a histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. Also functions as an acetyltransferase for nonhistone targets. Acetylates 'Lys-131' of ALX1 and acts as its coactivator in the presence of CREBBP. Acetylates SIRT2 and is proposed to indirectly increase the transcriptional activity of TP53 through acetylation and subsequent attenuation of SIRT2 deacetylase function. Acetylates HDAC1 leading to its inactivation and modulation of transcription. Can also mediate transcriptional repression. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. p300 antibody (mAb) (Clone NM11) was raised in a Mouse host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Immunocytochemistry, Immunofluorescence, Immunoprecipitation and Western blot, it has been shown to react with Human samples.

Aufreinigung: Protein A Chromatography

Immunogen: The antibody was raised against full-length p300 purified from human 293 cells.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Details zu p300

Target: p300 (EP300) (E1A Binding Protein P300 (EP300))

Andere Bezeichnung: p300

Molekulargewicht: 300 kDa

NCBI Accession: NP_001420

Pathways: p53 Signalweg, Notch Signalweg, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size