Histone 3 Antikörper (pSer10)

Produktnummer ABIN6972057

Der Ratte Monoklonal Anti-Histone 3-Antikörper wurde für WB, IF, ELISA und ICC validiert. Er ist geeignet, Histone 3 in Proben von Human zu detektieren.

Kurzübersicht für Histone 3 Antikörper (pSer10) (ABIN6972057)

Target: Histone 3 (H3) (Histone H3 (H3))

Reaktivität: Human

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser Histone 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunocytochemistry (ICC)

Klon: 6G8B7

Produktdetails anti-Histone 3 Antikörper

Bindungsspezifität: pSer10

Produktmerkmale: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. Interestingly, phosphorylation of Ser10 on histone H3 is involved in both transcription and cell division, two events requiring opposite alterations in the degree of chromatin compaction. Ser10 in the tail of histone H3 is strongly phosphorylated early in mitosis when chromosomes begin to condense, and during premature chromosome condensation induced in S-phase cells. Histone H3S10ph antibody (mAb) (Clone 6G8B7) was raised in a Rat host. It has been validated for use in ELISA, Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.

Aufreinigung: Protein G Chromatography

Immunogen: This antibody was raised against a peptide corresponding to amino acids 1-19 including phospho-serine 10 of human Histone H3.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Details zu Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Andere Bezeichnung: Histone H3

Molekulargewicht: 17 kDa

NCBI Accession: NP_003522