Histone 3 Antikörper (acLys9)

Produktnummer ABIN6972004

Dieses Anti-Histone 3-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Histone 3 in WB, IF, ChIP, DB, ICC und ChIP-seq. Geeignet für Human und Maus. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für Histone 3 Antikörper (acLys9) (ABIN6972004)

Target: Histone 3 (H3) (Histone H3 (H3))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Histone 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Immunocytochemistry (ICC), ChIP DNA-Sequencing (ChIP-seq)

Klon: 1B10

Produktdetails anti-Histone 3 Antikörper

Bindungsspezifität: acLys9

Produktmerkmale: Histone H3 is one of the core components of the nucleosome, the basic building block of chromatin. Histones are subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. Acetylation of Lys9 is associated with trascriptional activation of genes. Histone H3K9ac antibody (mAb) (Clone 1B10) was raised in a Mouse host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Dot blot, Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Human and Mouse samples, but it is predicted that it will react with a wide range of sample types.

Aufreinigung: Protein A Chromatography

Immunogen: The Histone H3 acetyl Lys9 antibody was raised against a peptide including acetyl-lysine 9 of human Histone H3.

Isotyp: IgG3

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Purified IgG in 0.1 M Tris, pH 8.0, 1 M NaCl with 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Referenzen für anti-Histone 3 Antikörper (ABIN6972004)

Kee, Thudium, Renner, Glastad, Palozola, Zhang, Li, Lan, Cesare, Poleshko, Kiseleva, Truitt, Cardenas-Diaz, Zhang, Xie, Kotton, Alysandratos, Epstein, Shi et al.: "SARS-CoV-2 disrupts host epigenetic regulation via histone mimicry." in: Nature, (2022) (PubMed).

Details zu Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Andere Bezeichnung: Histone H3

Molekulargewicht: 17

NCBI Accession: NP_003522