Histone H1.5 Antikörper (pSer17)

Produktnummer ABIN6971747

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch Histone H1.5 in WB und DB. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für Histone H1.5 Antikörper (pSer17) (ABIN6971747)

Target: Histone H1.5 (HIST1H1B)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Histone H1.5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Dot Blot (DB)

Produktdetails anti-Histone H1.5 Antikörper

Bindungsspezifität: pSer17

Produktmerkmale: The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Histone H1.5 is an H1 isotype expressed in somatic cells and is heavily phosphorylated during mitosis. Phosphorylation of H1.5 at serine 17 appears in prometaphase and disappears in telophase. The hyperphosphorylated form of H1.5 is mainly chromatin-bound in metaphase when chromatin is maximally condensed. Phosphorylation of H1.5 at serine 17 can be catalyzed by GSK-3. Histone H1.5S17ph antibody (pAb) was raised in a Rabbit host. It has been validated for use in Dot blot and Western blot, it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.

Aufreinigung: Affinity Purified

Immunogen: This Histone H1.5 phospho Ser17 antibody was raised against a peptide containing phospho Ser17 of human histone H1.5.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Details zu Histone H1.5

Target: Histone H1.5 (HIST1H1B)

Andere Bezeichnung: Histone H1.5

Molekulargewicht: 36 kDa

NCBI Accession: NP_005313