IL-31 Antikörper

Produktnummer ABIN6964030

Dieses Anti-IL-31-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von IL-31 in Neut. Geeignet für Human.

Kurzübersicht für IL-31 Antikörper (ABIN6964030)

Target: IL-31 (IL31) (Interleukin 31 (IL31))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser IL-31 Antikörper ist unkonjugiert

Applikation: Neutralization (Neut)

Klon: MT313

Produktdetails anti-IL-31 Antikörper

Spezifität: Native and recombinant human IL-31

Aufreinigung: Purified from in vitro cultures by protein G affinity chromatography.

Immunogen: Recombinant human IL-31

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Neutralization of human IL-31 bioactivity. It is recommended to establish the optimal antibody concentration for the assay system used. Neutralization Neutralization assay: Neutralization of human IL-31 induced IL-6 production (see graph). To determine the neutralizing capacity and ND50, mixtures of human recombinant IL-31 (50 ng/ ml) and various concentrations of mAb MT313 were added to human aortic endothelial cells that had been pre-stimulated with recombinant human IFN-γ (10 ng/mL). Following overnight incubation, cell supernatants were quantified in IL-6 ELISA. Neutralization dose 50 (ND50): MT313 neutralized the effect of human IL-31 at a ND50value of 0.1-0.6 μg/mL when 50 ng/mL of human IL-31 was present. ND50 is defined as the concentration of antibody that results in 50 % inhibition of the biological response under the experimental conditions specified.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: supplied at 1 mg/mL in PBS free of preservatives

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Haltbarkeit: 18 months

Details zu IL-31

Target: IL-31 (IL31) (Interleukin 31 (IL31))

Andere Bezeichnung: IL31

Gen-ID: 386653