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IL-12/IL-23 p40 Antikörper

Reaktivität: Human Neut Wirt: Maus Monoclonal MT3279H unconjugated
Produktnummer ABIN6964021
  • Target Alle IL-12/IL-23 p40 Produkte
    IL-12/IL-23 p40
    Reaktivität
    • 15
    • 10
    • 1
    Human
    Wirt
    • 15
    • 10
    Maus
    Klonalität
    • 25
    Monoklonal
    Konjugat
    • 7
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser IL-12/IL-23 p40 Antikörper ist unkonjugiert
    Applikation
    • 20
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Neutralization (Neut)
    Spezifität
    Native and recombinant p40 subunit of human IL-12 and IL-23
    Aufreinigung
    Purified from in vitro cultures by protein G affinity chromatography.
    Immunogen
    Recombinant human IL-23
    Klon
    MT3279H
    Isotyp
    IgG2b
  • Applikationshinweise
    Neutralization of human IL-12 and IL-23 bioactivity. It is recommended to establish the optimal antibody concentration for the assay system used. Neutralization Neutralization assay: Neutralization of human IL-12 and human IL-23 induced IFN-γ production (see graphs). To determine the neutralizing capacity and ND50 on IL-12 induced IFN-γ production, mixtures of various concentrations of mAb MT3279H, human recombinant IL-12 (10 ng/mL) and recombinant IL-1β (50 ng/mL) were added to human PBMC. For determination of the neutralizing capacity and ND50 on IL-23 induced IFN-γ production, mixtures of various concentrations of mAb MT3279H, human recombinant IL-23 (10 ng/ ml) and recombinant IL-18 (50 ng/mL) were added to human PBMC. Following overnight incubation, cell supernatants were quantified in IFN-γ ELISA. Neutralization dose 50 (ND50): MT3279H neutralized the effect of IL-12 and IL-23 at a ND50value of 0.2-0.5 μg/mL when 10 ng/mL of recombinant human IL-12 or IL-23 was present. ND50 is defined as the concentration of antibody that results in 50 % inhibition of the biological response under the experimental conditions specified.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    1 mg/mL
    Buffer
    supplied at 1 mg/mL in PBS free of preservatives
    Konservierungsmittel
    Without preservative
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
    Haltbarkeit
    18 months
  • Saghafian-Hedengren, Sohlberg, Theorell, Carvalho-Queiroz, Nagy, Persson, Nilsson, Bryceson, Sverremark-Ekström: "Epstein-Barr virus coinfection in children boosts cytomegalovirus-induced differentiation of natural killer cells." in: Journal of virology, Vol. 87, Issue 24, pp. 13446-55, (2014) (PubMed).

  • Target
    IL-12/IL-23 p40
    Andere Bezeichnung
    IL12/23 (p40) (IL-12/IL-23 p40 Produkte)
    Gen-ID
    3592, 3593
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