Interleukin 17a Antikörper (FITC)

Produktnummer ABIN6963909

Der Maus Monoklonal anti-Interleukin 17a Antikörper wird verwendet zum Nachweis von Interleukin 17a in Proben von Human, Rind (Kuh), Schwein, Hund, Schaf, Pferd und Non-Human Primate. Er wurde validiert für FACS.

Kurzübersicht für Interleukin 17a Antikörper (FITC) (ABIN6963909)

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Reaktivität: Human, Rind (Kuh), Schwein, Hund, Schaf, Pferd, Non-Human Primate

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Interleukin 17a Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS)

Klon: MT504

Produktdetails anti-Interleukin 17a Antikörper (FITC)

Spezifität: Native and recombinant IL-17A.

Kreuzreaktivität (Details): The antibody also cross-reacts with IL-17A from cow, sheep, horse, pig and dog.

Aufreinigung: Purified from in vitro cultures by protein G affinity chromatography.

Immunogen: Recombinant human IL-17A

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Detection of IL-17A producing cells by flow cytometry. 5 μL is recommended for staining of 1 million cells in a total volume of 50 μL. Detection of IL-17A by flow cytometry in human peripheral blood mononuclear cells (PBMC). Human PBMC were stimulated with PMA/ionomycin for 16 hours in the presence of Brefeldin A. The cells were then fixed and permeabilized using 4 % paraformaldehyde and saponin, and subsequently stained with anti-human IL-17A mAb MT504-FITC and anti-human IFN-γ-PE (right panel). The left panel depicts stimulated cells stained with matched isotype control antibodies. The dot plots derive from a lymphocyte gate. Flow cytometry was performed on a BD FACSVerse system.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: in PBS with 0.2 % BSA and 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Haltbarkeit: 18 months

Details zu Interleukin 17a

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Andere Bezeichnung: IL17A

Gen-ID: 3605, 282863, 100034142