CD8 alpha Antikörper (violetFluor™ 450)

Produktnummer ABIN7278540

Dieses Anti-CD8 alpha-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CD8 alpha in FACS. Geeignet für Human.

Kurzübersicht für CD8 alpha Antikörper (violetFluor™ 450) (ABIN7278540)

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD8 alpha Antikörper ist konjugiert mit violetFluor™ 450

Applikation: Flow Cytometry (FACS)

Klon: OKT8

Produktdetails anti-CD8 alpha Antikörper (violetFluor™ 450)

Aufreinigung: This monoclonal antibody preparation was purified from tissue culture supernatant via affinity chromatography. For In Vivo Ready™ (IVR) products, each preparation is also evaluated for endotoxin levels using the LAL assay. It is recommended to store the product undiluted at 4°C. Do not freeze.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: This antibody preparation has been pre-titrated and quality-tested for flow cytometry using an appropriate cell type. The antibody has been diluted for use at 5 μLper test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 uL. The number of cells within a sample should be determined empirically, but typically ranges between 1x10e5 to 1x10e8 cells. violetFluor™ 450 dye is excited by the violet (405 nm) laser and has a peak emission of 450 nm. The most common band pass filters for this dye are 440/40 or 450/50. violetFluor™ 450 can be used as an alternative for Pacific Blue®, BD Horizon™ V450 or eFluor® 450.

Kommentare:

5 uL (0.125 ug)/test

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 10 mM NaH2PO4, 150 mM NaCl, 0.09 % Sodium azide, 0.1 % gelatin, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: 2-8°C protected from light

Haltbarkeit: 12 months

Details zu CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Andere Bezeichnung: CD8a

Hintergrund: The OKT8 antibody is specific for the 32-34 kDa alpha chain of human CD8, known as CD8a or CD8 alpha. CD8a can form a homodimer (CD8 alpha-alpha), but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor for antigen recognition and subsequent T cell activation that is initiated upon binding of the T cell receptor (TCR) to antigen- bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck, facilitating intracel- lular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death of tumor cells, as well as cells infected by virus, bacteria or parasites. The OKT8 antibody is widely used as a phenotypic marker for CD8 on cytotoxic T cells, thymocytes, as well as on certain cell types that do not also express the TCR, including some NK cells and lymphoid dendritic cells. If used together with alternative antibodies Anti-Human CD8a clone RPA-T8 or Anti-Human CD8a clone Hit8a, the OKT8 antibody will not block binding of RPA-T8 or Hit8a.

Gen-ID: 925

UniProt: P01732

Pathways: T-Zell Rezeptor Signalweg