CD8 alpha Antikörper (violetFluor™ 450)

Produktnummer ABIN7278539

Der Ratte Monoklonal Anti-CD8 alpha-Antikörper wurde für FACS validiert. Er ist geeignet, CD8 alpha in Proben von Maus zu detektieren.

Kurzübersicht für CD8 alpha Antikörper (violetFluor™ 450) (ABIN7278539)

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD8 alpha Antikörper ist konjugiert mit violetFluor™ 450

Applikation: Flow Cytometry (FACS)

Klon: 53-6-7

Produktdetails anti-CD8 alpha Antikörper (violetFluor™ 450)

Aufreinigung: This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. It is recommended to store the product undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: This antibody preparation has been quality-tested for flow cytometry using mouse spleen cells, or an appropriate cell type (where indi- cated). The amount of antibody required for optimal staining of a cell sample should be determined empirically in your system. violet-FluorTM 450 dye is excited by the violet (405 nm) laser and has a peak emission of 450 nm. The most common band pass filters for this dye are 440/40 or 450/50. violetFluorTM 450 can be used as an alternative for Pacific Blue®, BD Horizon™ V450 or eFluor® 450.

Kommentare:

0.2 mg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 10 mM NaH2PO4, 150 mM NaCl, 0.09 % Sodium azide, 0.1 % gelatin, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: 2-8°C protected from light

Haltbarkeit: 12 months

Details zu CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Andere Bezeichnung: CD8a

Hintergrund: The 53-6.7 antibody reacts with the 32-34 kDa alpha subunit of mouse CD8, known as CD8a or CD8 alpha. CD8a can form a homodimer (CD8 alpha-alpha), but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor in antigen recognition and subsequent T cell activation that is initiated upon binding of the T cell receptor (TCR) to antigen- bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck, facilitating intracel- lular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death of tumor cells, as well as cells infected by virus, bacteria or parasites.The 53-6.7 antibody is widely used as a phenotypic marker for mouse CD8a expression on cytotoxic T cells, thymocytes, as well as on certain cell types that do not also express the TCR, including some NK cells and lymphoid dendritic cells.

Gen-ID: 12525

UniProt: P01731

Pathways: T-Zell Rezeptor Signalweg