Rekombinanter mRuby Antikörper

Produktnummer ABIN6953292

Produktdetails

Target: mRuby

Antikörpertyp: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reaktivität: Entacmaea quadricolor

Wirt: Alpaca

Expressionssystem: E.coli

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Immunofluorescence (IF), ELISA, Immunohistochemistry (IHC)

Verwendungszweck: Unlabelled anti-mRuby sdAb with ectopic Cysteine on N- and C-Term for maleimide conjugation.

Spezifität: MRUBY3 and mRUBY derivatives

Produktmerkmale: The anti-mRUBY single-domain antibody has the same specificity as the respective labeled variant, but can be purchased here as unconjugated version.

Aufreinigung: Produced in: E.coli

Klon: 1F8

Affinity: Extinction coefficient (1/M*cm): 20190

Anwendungsinformationen

Applikationshinweise: Uncoupled sdAb for doing maleimide conjugations. You can chose the position of the ectopic cysteine (N-terminal, C-terminal or both ends).
Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only.

Protokoll: Maleimide Labelling Protocol

• Remove TCEP and exchange buffer to 10 mM KPi, 300 mM NaCl, 2 mM EDTA, pH 6.0 by size exclusion chromatography
• Before labelling, add 1/10 vol 1M Tris/HCl pH 8.0
• On ice, add 1.5- to 5- fold molar excess of fluorophore as maleimide
• Quickly vortex
• Overlay with Argon
• Place on ice for 90 min
• Separate excess of dye from the labelled sdAb e.g. by size exclusion chromatography

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Unconjugated sdAbs are lyophilized from 10 mM KPi pH 6.0,150 mM NaCl, 1 mM EDTA. Reconstitute with 250 µL pure water. Use unconjugated sdAbs immediately after reconstitution.

Buffer: lyophilized from 10 mM KPi, 300 mM NaCl, 2 mM EDTA, pH 6.0

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Up to 3 months store at -20 °C. Up to 12 months store at -80 °C or below

Haltbarkeit: 12 months

Antigendetails

Target: mRuby