Rekombinanter mRuby Antikörper (Alexa Fluor 647)

Produktnummer ABIN6953280

Produktdetails

Target: mRuby

Antikörpertyp: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reaktivität: Entacmaea quadricolor

Wirt: Alpaca

Expressionssystem: E.coli

Klonalität: Monoklonal

Konjugat: Alexa Fluor 647

Applikation: Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Verwendungszweck: Camelid sdAb anti-mRuby conjugated with Alexa647, Clone 1F8

Spezifität: Recognizes mRuby, mRuby3 and common mRuby derivates.

Produktmerkmale: A dye conjugated alpaca single-domain antibody (sdAb), also referred to as VHH or nanobody. It is in the range of 15 kDa and 3 nm in molecular weight and size respectively. This means that sdAbs are ten times lighter and up to 5x smaller than a conventional IgG molecule. They can position a fluorophore up to 20 nm closer to the intended target than using conventional primary-secondary antibody complex detection.

Aufreinigung: Produced in: E.coli

Klon: 1F8

Labeling Ratio: Two site-specifically conjugated fluorophores per sdAb.

Anwendungsinformationen

Applikationshinweise: Recommended dilution 1:500

Kommentare:

Two site-specifically coupled fluorophores per molecule. The reagent can therefore simultaneously target two fluorophores to your protein of interest, which results in enhanced image brightness. Owing to the small size of the sdAb, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, this sdAb can thus improve the localization accuracy by 10-15 nm. Both features - enhanced brightness and precise fluorophore placement - renders this product superior tools for all microscopy techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with 200 µL of 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted with 200 µl, the concentration of single-domain antibody is 2.5 µM

Buffer: The single sdAb clone was lyophilized from PBS pH 7.4, containing 2% BSA.

Konservierungsmittel: Without preservative

Handhabung: Protect from light!

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Up to 3 months store at -20 °C. Up to 12 months store at -80 °C or below

Haltbarkeit: 12 months

Antigendetails

Target: mRuby