Rekombinanter Blue Fluorescent Protein Antikörper (Alexa Fluor 647)

Produktnummer ABIN6953240

Produktdetails anti-Blue Fluorescent Protein Antikörper (Alexa Fluor 647)

Target: Blue Fluorescent Protein (BFP)

Antikörpertyp: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reaktivität: Entacmaea quadricolor

Wirt: Alpaca

Expressionssystem: E.coli

Klonalität: Monoklonal

Konjugat: Dieser Blue Fluorescent Protein Antikörper ist konjugiert mit Alexa Fluor 647

Applikation: Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Verwendungszweck: Camelid sdAb anti-TagBFP conjugated with Alexa647, Clone 1H7

Spezifität: Recognizes mTagBFP, mKate, mKate2, mTagRFP, mTagRFP657 and most common fluorescent proteins deriving from Entacmaea quadricolor

Kreuzreaktivität (Details): Does not cross-react with common GFP- or dsRed derivatives.

Produktmerkmale: A dye conjugated alpaca single-domain antibody (sdAb), also referred to as VHH or nanobody. It is in the range of 15 kDa and 3 nm in molecular weight and size respectively. This means that sdAbs are ten times lighter and up to 5x smaller than a conventional IgG molecule. They can position a fluorophore up to 20 nm closer to the intended target than using conventional primary-secondary antibody complex detection.

Aufreinigung: The antibody produced in E.coli is derived from a single-domain antibody (sdAb) that recognizes fluorescent proteins deriving from Entacmaea quadricolor, such as mTagBFP, mTagRFP, mTagRFP657, and mKate with high affinity and specificity.

Klon: 1H7

Labeling Ratio: a single fluorophore is coupled to exactly one sdAb

Anwendungsinformationen

Applikationshinweise: Recommended dilution 1:500

Kommentare:

Each fluorophore is coupled to exactly one sdAb, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a high coupling efficiency of > 95% guarantees a highly linear relation between target molecule number and fluorescent intensity. This enables you to directly count your target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: 5 μM

Buffer: 5 μM fluorescently labeled sdAb in buffered saline, 50 % glycerol, 0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light!

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Up to 3 months store at -20 °C. Up to 12 months store at -80 °C or below

Haltbarkeit: 12 months

Details zu Blue Fluorescent Protein

Target: Blue Fluorescent Protein (BFP)

Andere Bezeichnung: TagBFP (BFP Produkte)