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Histone 3 Antikörper (H3K9me3)

Der Kaninchen Polyklonal Anti-Histone 3-Antikörper wurde für WB, IF, ELISA, ChIP, DB, ChIP-seq, CUT&Tag und PeA validiert. Er ist geeignet, Histone 3 in Proben von Human zu detektieren.
Produktnummer ABIN6952362

Kurzübersicht für Histone 3 Antikörper (H3K9me3) (ABIN6952362)

Target

Alle Histone 3 (H3) Antikörper anzeigen
Histone 3 (H3) (Histone H3 (H3))

Reaktivität

  • 1352
  • 910
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  • 41
  • 39
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  • 34
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Human

Wirt

  • 1156
  • 255
  • 12
  • 5
Kaninchen

Klonalität

  • 1074
  • 353
  • 1
Polyklonal

Konjugat

  • 816
  • 72
  • 47
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  • 1
Dieser Histone 3 Antikörper ist unkonjugiert

Applikation

  • 1028
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Western Blotting (WB), Immunofluorescence (IF), ELISA, Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Tagmentation (CUT&Tag), Peptide Array (PeA)

Güteklasse

ChIP-seq Grade
  • Bindungsspezifität

    • 56
    • 50
    • 48
    • 44
    • 43
    • 37
    • 37
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    • 35
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    H3K9me3

    Spezifität

    Polyclonal antibody raised in rabbit against the region of histone H3 containing the trimethylated lysine 9 (H3K9me3), using a KLH-conjugated synthetic peptide.

    Kreuzreaktivität

    Maus, Yeast (Saccharomyces cerevisiae)

    Kreuzreaktivität (Details)

    broad range

    Aufreinigung

    Peptide affinity purified

    Immunogen

    synthetic peptide
  • Applikationshinweise

    ChIP/ChIP-seq 1 μg:ChIP
    ELISA 1:1,000
    Dot Blot 1:20,000
    Peptide array 1:5,000
    WB 1:1,000
    IF 1:250

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    0.9 μg/μL

    Buffer

    PBS, 0.05 % azide, 0.05 % ProClin300

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    -20 °C
  • Target

    Histone 3 (H3) (Histone H3 (H3))

    Andere Bezeichnung

    Histone 3

    Hintergrund

    Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Trimethylation of histone H3K9 is associated with inactive genomic regions, satellite repeats and ZNF gene repeats.

    UniProt

    P68431
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